DAPI, DIHYDROCHLORIDE
4',6-diamidino-2-phenylindole dihydrochloride
CAS: 28718-90-3
Molecular Formula: C16H17Cl2N5
DAPI, DIHYDROCHLORIDE - Names and Identifiers
| Name | 4',6-diamidino-2-phenylindole dihydrochloride |
| Synonyms | DAPI CCRIS 8836 -Cellstain-Dapi DAPI, DIHYDROCHLORIDE CELLSTAIN- DAPI SOLUTION 4',6-DIAMINO-2-PHENYLINDOLE DIHYDROCHLORIDE 4',6-diamidino-2-phenylindole dihydrochloride 4',6-DIAMIDINO-2-PHENYLINDOLE*DIHYDROCHL ORIDE HYDRA 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide 2-(4-AMIDINOPHENYL)-6-INDOLECARBAMIDINEDIHYDROCHLORIDE 2-phenylindole-4',6-dicarboxamidine dihydrohydrochloride (hydrate) 2-Phenylindole-4',6-dicarboxamidine dihydrohydrochloride (hydrate) 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride |
| CAS | 28718-90-3 |
| EINECS | 249-186-7 |
| InChI | InChI=1/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H |
| InChIKey | FPNZBYLXNYPRLR-UHFFFAOYSA-N |
DAPI, DIHYDROCHLORIDE - Physico-chemical Properties
| Molecular Formula | C16H17Cl2N5 |
| Molar Mass | 350.25 |
| Melting Point | >300°C (dec.) |
| Boling Point | 545.4°C at 760 mmHg |
| Flash Point | 283.6°C |
| Solubility | Soluble in water |
| Vapor Presure | 5.96E-12mmHg at 25°C |
| Appearance | Yellow solid |
| Color | Clear |
| Maximum wavelength(λmax) | ['358 nm, 342 nm'] |
| BRN | 4894417 |
| PH | 4.0-5.0 (10g/l, H2O, 20℃) |
| Storage Condition | 2-8°C |
| Stability | PROTECT FROM LIGHT! Stable for 2 years as supplied. Solutions in DMSO may be aliquoted and stored at -20°C for up to 3 months. |
| MDL | MFCD00012681 |
| Use | This product is for scientific research only and shall not be used for other purposes. |
DAPI, DIHYDROCHLORIDE - Risk and Safety
| Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R20/21/22 - Harmful by inhalation, in contact with skin and if swallowed. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S24/25 - Avoid contact with skin and eyes. S22 - Do not breathe dust. S37/39 - Wear suitable gloves and eye/face protection |
| WGK Germany | 3 |
| RTECS | NL5995050 |
| FLUKA BRAND F CODES | 3-8-10-21 |
| HS Code | 29339900 |
DAPI, DIHYDROCHLORIDE - Reference
| Reference Show more | 1. Zhang Xiaohan, Zhao Jiang, Han Ying, etc. Retarding senescence of female Drosophila melanogaster by Phloretin [J]. Modern food science and technology, 2020, v.36;No.247(03):15-22 172. 2. Guan, X., et al. "High effective drug delivery polymeric micells with charge-switching and cyclic rgd targeting for treatment of gliobastoma." DIGEST JOURNAL OF NANOMATERIALS AND BIOSTRUCTURES 10.3 (2015): 789-798. 3. Guan, Xingang, et al. "Cyclic RGD targeting nanoparticles with pH sensitive polymer-drug conjugates for effective treatment of melanoma." RSC Advances 4.98 (2014): 55187-55194.DOI https://doi.org/10.1039/C4RA08537J 4. Guan, Xingang, et al. "cRGD targeted and charge conversion-controlled release micelles for doxorubicin delivery." Rsc Advances 5.29 (2015): 22957-22964.10.1039/C4RA14368J 5. Kunhong Yu, Jingwen Hou, Zhu Jin, Keqin Wu Shaohui Xu, Ning Yang, Yuanyuan Shen, Tingting Tang, Shengrong Guo, A cochlear implant loaded with dexamethasone and coated with hyaluronic acid to inhibit fibroblast adhesion and proliferation, Journal of Drug D 6. [IF=4.207] Kongtong Yu et al."Role of Four Different Kinds of Polyethylenimines (PEIs) in Preparation of Polymeric Lipid Nanoparticles and Their Anticancer Activity Study."J Cancer. 2016; 7(7): 872-882 7. [IF=3.981] Kunhong Yu et al."A cochlear implant loaded with dexamethasone and coated with hyaluronic acid to inhibit fibroblast adhesion and proliferation."J Drug Deliv Sci Tec. 2018 Aug;46:173 8. [IF=2.819] Qu Na et al."Docetaxel-loaded human serum albumin (HSA) nanoparticles: synthesis, characterization, and evaluation."Biomed Eng Online. 2019 Dec;18(1):1-14 9. [IF=6.038] Ge Gaoran et al."Punicalagin ameliorates collagen-induced arthritis by downregulating M1 macrophage and pyroptosis via NF-κB signaling pathway."Sci China Life Sci. 2021 Jun;:1-16 10. [IF=4.539] Dong-xue Ren et al."pH/redox dual response nanoparticles with poly-γ-glutamic acid for enhanced intracellular drug delivery."Colloid Surface A. 2019 Sep;577:412 11. [IF=2.466] Jianyun Cao et al."Puerarin promotes the osteogenic differentiation of rat dental follicle cells by promoting the activation of the nitric oxide pathway."Tissue Cell. 2021 Dec;73:101601 12. [IF=1.466] Zhibing Qiu et al."LncRNA AC061961.2 overexpression inhibited endoplasmic reticulum stress induced apoptosis in dilated cardiomyopathy rats and cardiomyocytes via activating wnt/β-catenin pathway."J Recept Sig Transd. 2021;41(5):494-503 13. [IF=13.273] Xuefang Hao et al.Biomimetic and responsive nanoparticles loading JQ1 for dual-targeting treatment of vascular restenosis via multiple actions.Chem Eng J. 2021 Nov;:133452 14. [IF=6.953] Qihong Zhang et al."Preparation, physicochemical and pharmacological study of 10-hydroxycamptothecin solid dispersion with complexation agent - xylan-nonanoic acid amphiphilic conjugates."Int J Biol Macromol. 2022 Apr;204:224 15. [IF=9.933] Zerui Wu et al."Regulating Macrophage Polarization in High Glucose Microenvironment Using Lithium-Modified Bioglass-Hydrogel for Diabetic Bone Regeneration."Advanced Healthcare Materials 16. [IF=30.849] Jinjin Shi et al."Intracellular self-assembly driven uninterrupted ROS generator augments 5-aminolevulinic acid-based tumor therapy."ADVANCED MATERIALS |
DAPI, DIHYDROCHLORIDE - Introduction
DAPI staining solution is a staining solution suitable for nuclear staining of all common cells and tissues. It can be directly used to fix cells or tissues for nuclear staining. DAPI is a blue fluorescent dye that can penetrate cell membranes. Combined with double-stranded DNA, it can produce fluorescence more than 20 times stronger than DAPI itself. Compared with EB(ethidium bromide), the staining sensitivity for double-stranded DNA is many times higher. DAPI staining is often used to detect apoptosis. After staining, it is observed by fluorescence microscope or flow cytometry. DAPI is also commonly used for common nuclear staining and double-stranded DNA staining in certain specific situations.
Last Update：2022-10-16 17:24:16
DAPI, DIHYDROCHLORIDE - Reference Information
| biological field application | Nuclear apoptosis say; targeting drug to cerebral neuron; treating amyloidosis,neurodegenerative diseases,Alzheimer's disease,Down's syndrome,type II diabetes; monitoring bioaerosols |
| biological activity | DAPI is an intercalating agent containing specific AT sequence DNA, which can adhere to the small groove area of the double helix of DNA like a Hoechst dye. Compared with EB(ethidium bromide), the staining sensitivity for double-stranded DNA is many times higher. Although DAPI cannot pass through the living cell membrane, it can penetrate the disturbed cell membrane and stain the nucleus. DAPI has a high photobleaching tolerance level and can be used to detect yeast mitochondrial DNA, chloroplast DNA, viral DNA,microplasm DNA and chromosomal DNA. |
| operation attention | 1. DAPI has certain irritation to human body, please pay attention to proper protection. 2. fluorescent dyes have quenching problems. it is recommended to complete the detection as much as possible on the same day after dyeing. 3. anti-fluorescence quenching sealing solution can be used to slow down fluorescence quenching. 4. for your safety and health, please wear experimental clothing and disposable gloves. |
| use | DAPI fluorescent dye can penetrate cell membrane and bind to nuclear double-stranded DNA to play a labeling role, produce fluorescence more than 20 times stronger than itself, and have high EB sensitivity to double-stranded DNA staining. Under the microscope, the fluorescence efficiency of blue fluorescent cells is almost 100%, and it has no toxic side effects on living cells. It is often used for the detection of apoptosis. After staining, it is observed by fluorescence microscope or flow cytometry. It is also used for ordinary nuclear staining and double-stranded DNA staining under certain circumstances. For stained DNA in agarose gel, DAPI is several times more sensitive to bromohexidine bromide. It can be used for DNA The light footprint mapping method, in the smearing application, detects the annealed probe through the unique observable double-stranded complex, as well as the use of flow cytometry to study DNA changes and analyze DNA content in the process of apoptosis; DAPI coloring has been shown to be a sensitive and specific detection method for mycoplasma DAPI staining is often used for apoptosis detection. After staining, it is observed by fluorescence microscope or flow cytometry. DAPI is also commonly used for common nuclear staining and double-stranded DNA staining in certain specific situations. a fluorescent dye combined with DNA rich in A- T sequence. DAPI is a cell-permeable DNA-binding dye which binds preferentially to DNA rich in adenine and thymine. It is used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes and is also useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. DAPI can also be used to detect mycoplasma and in simple retrograde double-labelling procedures for studying axonal branching employing Evans Blue and Primulin. DAPI is a reversible inhibitor of S-adenosyl-L-methionine decarboxylase and KAO (diamine oxidase). |
Last Update：2024-04-09 21:11:58
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View History
DAPI, DIHYDROCHLORIDE
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碘化钾汞
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APY0201